Evaluation of 150µm ID Packed-Tip Columns for Qunatitative Peptide Analysis by LC-MS/MS

Stanley Durand, Helena Svodoba, Amanda Berg, Gary A. Valaskovic
¹New Objective, Inc., Woburn, MA; ²Milestone Development Services, Newtown, PA

Optimization of Nanospray Voltage and Spray Stability: Impact on Chromatographic Peak Area for Analyte Quantitation

Amanda Berg¹, Mike S. Lee², Gary A. Valaskovic^1
1New Objective, Inc., Woburn, MA; ²Milestone Development Services, Newtown, PA

Fundamental Characteristics of Small Molecule Analysis Using Ultra-Low Dynamic Flow

Ben Ngo¹; Shaoxia Yu²; Mike S. Lee³; Jing-Tao Wu²; Gary Valaskovic^1
1New Objective, Inc., Woburn, MA; ²Millennium Pharmaceuticals, Inc., Cambridge, MA; ³Milestone Development Services, Newtown, PA

Improving Nanobore Column Duty Cycle via Trap-Column Injection: Evaluating the Effect of Trap-Column Injection Flow Rate on Analytical Separation

Carla Marshall-Waggett, Helena Svobodova, Amanda Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Evaluation of 150µm ID Packed-Tip Columns for Qunatitative Peptide Analysis by LC-MS/MS

Stanley Durand, Helena Svodoba, Amanda Berg, Gary A. Valaskovic
¹New Objective, Inc., Woburn, MA; ²Milestone Development Services, Newtown, PA

Optimization of Nanospray Voltage and Spray Stability: Impact on Chromatographic Peak Area for Analyte Quantitation

Amanda Berg¹, Mike S. Lee², Gary A. Valaskovic^1
1New Objective, Inc., Woburn, MA; ²Milestone Development Services, Newtown, PA

Fundamental Characteristics of Small Molecule Analysis Using Ultra-Low Dynamic Flow

Ben Ngo¹; Shaoxia Yu²; Mike S. Lee³; Jing-Tao Wu²; Gary Valaskovic^1
1New Objective, Inc., Woburn, MA; ²Millennium Pharmaceuticals, Inc., Cambridge, MA; ³Milestone Development Services, Newtown, PA

Increased Sample Loading Capacity For Peptide Analysis by LC-MS/MS Using 150 µm ID Packed-Tip Columns

Amanda Berg, Helena Svobodova, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Evaluation of Nanospray Voltage and Spray Stability and their Impact on Chromatographic Peak Area

Gary A. Valaskovic¹, Mike S. Lee², Amanda Berg^1
1New Objective, Inc., Woburn, MA; ²Milestone Development Services, Newtown, PA

Flow Rate, Concentration and Saturation: Investigating the Fundamentals of Sample-Trapped Column Injection

Carla Marshall-Waggett, Amanda Berg, Helena Svobodova, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Optimization of Sample Loading Capacity for Absolute Peptide Quantification by Nanobore LC-MS/MS

Amanda Berg¹, Gary A. Valaskovic¹, Chuck Witkowski², Jeremy L. Norris², Mike S. Lee^3
1New Objective, Woburn, MA; ²Protein Discovery, Knoxville, TN; ³Milestone Development Services, Newtown, PA

Nanobore RPHPLC: Determining The Role of Selectivity in Method Development

Amanda Berg, Carla Marshall-Waggett, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

On-line Microfluidic Extraction Enables Highly Efficient and Sensitive Direct Elution from Dried Blood Spots

Gary A. Valaskovic¹; Christopher A. Evans²; Chester L. Bowen^2
1New Objective, Inc., Woburn, MA; ²GlaxoSmithKline, King Of Prussia, PA

Flow Rate, Concentration and Saturation: Investigating the Fundamentals of Sample-Trapped Column Injection

Carla Marshall-Waggett, Amanda Berg, Helena Svobodova, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

On-line Microfluidic Extraction Enables Highly Efficient and Sensitive Direct Elution from Dried Blood Spots

Gary A. Valaskovic¹; Christopher A. Evans²; Chester L. Bowen^2
1New Objective, Inc., Woburn, MA; ²GlaxoSmithKline, King Of Prussia, PA

Advances in Capillary Liquid Chromatography for High-Throughput Top Down Proteomics

Adam D. Catherman¹, John C. Tran¹, Lee Sawdey³, Kenneth R. Durbin¹, Adaikkalam Vellaichamy², Gary Valaskovic³, Neil L. Kelleher^1,2
1Department of Chemistry, ²The Institute for Genomic Biology, University of Illinois, ³New Objective, Inc

Bridging the Gap Between Nanospray and ESI with Capillary Spray

Amanda Berg, Carla Marshall-Waggett, Gary Valaskovic
New Objective, Inc., Woburn, MA

Optimization of Sample Loading Capacity for Absolute Peptide Quantification by Nanobore LC-MS/MS

Amanda Berg¹, Gary A. Valaskovic¹, Chuck Witkowski², Jeremy L. Norris², Mike S. Lee^3
1New Objective, Woburn, MA; ²Protein Discovery, Knoxville, TN; ³Milestone Development Services, Newtown, PA

Nanobore RPHPLC: Determining The Role of Selectivity in Method Development

Amanda Berg, Carla Marshall-Waggett, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Novel and Robust ESI Source for Efficient Bioanalytical Workflowsat Capillary LC Flow Rates

Amanda Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

From Sample to Spray: High Performance Workflow for Top-Down Protein Analysis

¹Chuck Witkowski, ¹Jeremy Norris, ²Gary Valaskovic, ³Top-Down Development Team, ³Neil Kelleher
¹Protein Discovery, Inc., Knoxville, TN; ²New Objective, Inc., Woburn, MA; ³Center for Top-Down Proteomics, University of Illinois, Champagne-Urbana

Towards Robust Quantitative MRM Plasma Analysis using Nanobore Liquid Chromatogrpahy Through Improves Nanoelectrospray Performance

Susan E. Abbatiello¹, Amanda Berg², Gary Valaskovic², Steven A. Carr ^1
1The Broad Institute, Cambridge, MA; ¹New Objective, Woburn, MA

Minimization of Atmospheric Background Contaminants in Nanoelectrospray: Identification and Optimization

Benjamin Ngo, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Fundamental Studies on the Electrophoretic Enrichment of Peptides in Clinical Samples for Quantification using nanoLC-MS/MS

Jeremy L. Norris¹, Gary Valaskovic², Mike S. Lee³, Chuck Witkowski^1
1Protein Discovery, Inc., Knoxville, TN; ²New Objective Inc, Woburn, MA; ³Milestone Development Services, Newtown, PA
Not available for download

Top Down Proteomics Using Online Polymer Reversed Phase (PLRP) Nanocapillary-LC-Coupled Fourier Transform Mass Spectrometry

Adaikkalam Vellaichamy¹, John Kellie¹, John C. Tran¹, Ji Eun Lee¹ Jaeyun Sung¹,
Nathan D. Price¹, Gary Valaskovic², Neil L. Kelleher^1
1University of Illinois, Urbana, IL; ²New Objective Inc, Woburn, MA

Comparison of Chromatographic Media for Nano ESI LC-MS/MS: Applications to Biomarker Discovery

Michael Gardner¹, Megan Rowland², Jonathan L. Bundy¹, James Stephenson¹, Gary Valaskovic³, Mike S. Lee^4
1Research Triangle Institute, RTP, NC; ²RTI International, Cary, NC; ³New Objective Inc, Woburn, MA; ⁴Milestone Development Services, Newtown, PA
Not available for download

Ruggedness of Nanobore LCMS for Qualitative and Quantitative Biomarker Analysis using an Automated Emitter Positioning and Rinsing System

Amanda Berg, Carla Marshall-Waggett, Gary Valaskovic
NewObjective Inc, Woburn, MA

Improved Throughput for Clinical Proteomics Using a Dual Channel NanoLC-Nanospray Source

Cheryl F. Lichti¹, Benjamin Ngo², Marjorie N. Case¹, R.Reid Townsend¹, Gary A. Valaskovic^2
1Washington University School of Medicine, St. Louis, MO; ²New Objective Inc, Woburn, MA

Comparison of Conventional, Sub-2-µm, and Superficially Porous (Fused-Core) Particles for Long and Short Gradient Nanobore LC/MS

Robert Moody¹, Gary A. Valaskovic², Mike S. Lee^3
1MAC-MOD Analytical, Chadds Ford, PA; ²New Objective Inc, Woburn, MA; ³Milestone Development Services, Newtown, PA

Temperature Control Effects in Packed Emitter Nanobore LC-MS/MS for Protein Digest Analysis

Mike S. Lee¹, Lee Swadey², Amanda Berg², Gary A. Valaskovic^2
1Milestone Development Services, Newtown, PA; ²New Objective Inc, Woburn, MA

Spatial Mapping of Analyte Ion Intensity of Nanoelectrospray in the Plume-Inlet Region

Gary A. Valaskovic¹, Mike S. Lee^2
1New Objective Inc, Woburn, MA; ²Milestone Development Services, Newtown, PA

High Sensitivity Nanoscale Multidimensional LC/MS Analysis of Phosphopeptides

Scott Ficarro¹, Yi Zhang², Feng Zhou¹, Bob Cardoza¹, Guillame Adelmont¹, Manor Askenazi², Amanda Berg³, Gary A. Valaskovic³, Jared Marto^1
1Dana-Farber Cancer Institute, Boston, MA; ²Dana-Farber Cancer Institute and Hebrew University, Boston, MA; ³New Objective Inc, Woburn, MA
Not available for download

Imaging of Nanoelectrospray Spray Current with an Automated Digital Control Positioning System

Gary A. Valaskovic¹, Mike S. Lee^2
1New Objective, Woburn, MA; ²Milestone Development Services,Newtown, PA

Nanoelectrospray Ruggedness for Qualitative and Quantitative Biomarker Analysis Using an Automated Emitter Positioning and Rinsing System

Amanda Berg, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Minimization of Atmospheric Background Contaminants in Off- and Online Nanoelectrospray: Identification and Optimization

Benjamin Ngo, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Optimization of Nanoelectrospray with Plasma DerivedSamples for Qualitative and Quantitative Biomarker Analysis

Jeffrey Wynn, Amanda Berg, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Performance Comparison of Standard Electrospray vs. Nanospray for the Quantification of Nucleopeptides and their Phosphorolated Metabolites

John D. Lennon¹, Ken Lewis¹, Gary A Valaskovic^2
1OpAns, LLC, Durham, NC; ²New Objective Inc, Woburn, MA
Not available for download

Optimization of Novel Fused-Core Silica Particle Nanobore Columns Using a Pump-Switching Motif for Mass Spectrometry

Robert Moody¹; Gary A. Valaskovic²; Mike S. Lee^3
1MAC-MOD Analytical, Chadds Ford, Pennsylvania; ²New Objective, Inc., Woburn, MA; ³Milestone Development Services, Newtown, PA

High-Sensitivity Nanocapillary Chromatography Combined With Active Background Ion Reduction Increases Signal-to-Noise Ratios, Sensitivity,and Overall System Performance

John M. Neveu¹, Bogdan A. Budnik¹, Gary A. Valaskovic², William S. Lane^1
1Harvard University, Cambridge, MA; ²New Objective, Inc., Woburn, MA

Maximizing Performance of sub-2 µm Packed Nanobore LC Columns: Minizing Extra-Column Variance

Carla J . Marshall-Waggett, Amanda L. Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Investigation of Elastomer Core Compression Effect on Minimizing Trapping Column Band Broadening

Amanda L. Berg, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Measurement of Testosterone in Human Urine by Direct Injection Using nano-Pump Switching with Nanospray Tandem Mass Spectrometry

Daniel Magiera¹, Mike S. Lee², Gary A. Valaskovic^3
1Molecular MS Diagnostics, LLC, Cranston, RI; ²Milestone Development Services, Newtown, PA; ³New Objective, Inc., Woburn, MA
Not available for download

Imaging of Nanoelectrospray Spray Current with an Automated Digital Control Positioning System

Gary A. Valaskovic¹, Mike S. Lee^2
1New Objective, Woburn, MA; ²Milestone Development Services,Newtown, PA

Improving the Sensitivity of LC/MS Assays for Low-Abundance Protein Biomarkers: Bridging the Gap with ELISA

Lee W. Ott¹, Michael J. Berna¹, Gary A. Valaskovic², Bradley L. Ackermann^1
1Eli Lilly & Co., Greenfield, IN; ²New Objective, Inc., Woburn, MA
Not available for download

Correlating Extended Dynamic Range with Nanospray Flow Rate Measurement for Optimal ESI-MS Performance

Carla J. Marshall-Waggett, Christopher J. Toher, Gary A. Valaskovic
New Objective, Woburn, MA

Inline Electrospray Current Conductivity Detection for Characterization of Mobile Phase Composition and Gradient Delay

Adam W. Perala, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Efficient Column-to-Column Coupling with Multiple Columns for Enhanced nLC-ESI-MS Separation

Christopher Toher, Adam W. Perala, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

A Novel Fused-core Silica Particle Based Nanobore Column for Ultra-high Performance Nanoelectrospray Mass Spectrometry

Robert Moody¹; Gary A. Valaskovic²; Mike S. Lee^3
1MAC-MOD Analytical, Chadds Ford, Pennsylvania, ²New Objective, Inc., Woburn, MA, ³Milestone Development Services, Newtown, PA
Not available for download

Ultra-Performance Peptide Analysis via Nanobore LC-ESI-MS with Sub-2µm Particles

John M. Neveu¹, William S. Lane¹, Gary A. Valaskovic^{2 1}Harvard University, Cambridge, MA;
²New Objective, Inc., Woburn, MA

Digitized Nanobore LC-MS: An Automated Control System with Emitter Divert for Column-Switching

Mike S. Leesup>1, Gary A. Valaskovic^{2 1}Milestone Development Services, Newtown, PA, ²New Objective, Inc., Woburn, MA

Evaluation of Fluoropolymer Frit Performance in High-pH Nano-ESI Applications

Jeffrey Wynn, Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective Inc, Woburn, MA

On-line 1D and 2D nanoLC-ESI-MS using 10-µm-i.d. Porous Layer Open Tubular Polystyrene-divinylbenzene Columns for Ultrasensitive Proteomic Analysis

Quanzhou Luo¹, Guihua Yue¹, Gary A. Valaskovic², Ye Gu¹, Dongdong Wang¹, Shiaw-Lin Wu¹, Barry L. Karger^1
1The Barnett Institute, Northeastern University, Boston, MA, ²New Objective, Inc., Woburn, MA
Not available for download

Inline Electrospray Current Conductivity Detection for Characterizing Mobile Phase Composition and Gradient Delay

Gary A. Valaskovic, Adam W. Perala, Christopher J. Toher
New Objective Inc, Woburn, MA

Long- & Mixed-Column Nanobore Chromatography for Complex Proteomic Analysis

Christopher J. Toher, Adam W. Perala, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Examination of Nanobore Capillary Trapping Column Geometries for Analysis of Peptide Mixtures

John M. Neveu¹, Christopher J. Toher², William S. Lane¹, Gary A. Valaskovic^2
1Harvard University, Cambridge, MA   ²New Objective Inc, Woburn, MA

Rapid Nanobore LC-ESI-MS Peptide Analysis Using Sub-3 µm-Diameter Resins

Adam W. Perala, Christopher J. Toher, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

John M. Neveu¹, Christopher J. Toher², William S. Lane¹, Gary A. Valaskovic^2
1Harvard University, Cambridge, MA   ²New Objective Inc, Woburn, MA

High-Pressure, Transparent Microfluidic Coupling Method for Nanobore LC-MS

Jeffrey J. Wynn, Christopher J. Toher, Adam W. Perala, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Digitized Nanobore LC-MS Control System with Integrated Emitter Divert & Rinse for Improved Nanospray Performance

James L. Stephenson¹, Jonathan L. Bundy¹, Gary A. Valaskovic², Mike S. Lee^3
1RTI International, Research Triangle Park, NC   ²New Objective Inc, Woburn, MA   ³Milestone Development Service, Inc., Newtown, PA

Offline Nano-ESI Phosphopeptide Analysis with TiO₂, and ZrO₂ Wall-Coated Trap'nTips™

Christopher J. Toher¹, Adam W. Perala¹, Ashok K. Shukla², Gary A. Valaskovic^1
1New Objective Inc, Woburn, MA, ²Glygen, Inc., Columbia, MD

Offline Nano-ESI Phosphopeptide Analysis with Carbon, TiO₂, and ZrO₂
Wall-Coated Trap'nTips™

Christopher J. Toher¹, Adam W. Perala¹, Ashok K. Shukla², Gary A. Valaskovic^1
1New Objective Inc, Woburn, MA, ²Glygen, Inc., Columbia, MD

PicoClear™ Union Technology Applications for Nano ESI-MS Molecules

Carla J. Marshall-Waggett, Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Evaluation of Fluoropolymer Frit Performance in High-pH Nano-ESI Applications

Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Automated Orthogonal ESI Control System with Optimized Plume-Inlet Positioning

Mike S. Lee ¹, Gary A. Valaskovic^2
1Milestone Development Services, Inc., ²New Objective, Inc., Woburn, MA

Sample Purification for Static Nanospray MS Using Wall-Coated Pipette Trap'nTips™

Christopher J. Toher ¹, Adam W. Perala ¹, Ashok K. Shukla ², Gary A. Valaskovic^1
1New Objective Inc, Woburn, MA, ²Glygen, Inc., Columbia, MD

Enhanced Nanobore LC-MS Using Methanol Gradient Elution with Peptide Mixtures

Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

TIC-Mediated Flow Rate Variation for Flow-Injection NanosprayAnalysis

Carla J. Waggett¹, David Neyer², Adam W. Perala¹, Christopher J. Toher¹, Gary A. Valaskovic^1
2New Objective, Inc., Woburn, MA, ²Eksigent Technologies, Livermore, CA

Charged Droplet Solvent Extraction from Surfaces: Desoprtion Electrospray Ionization of Small Drug Molecules

Gary A. Valaskovic¹, Mike S. Lee^2
1New Objective Inc, Woburn, MA, ²Milestone Development Services, Inc.

Automated ESI Control for Variable Flow Nanobore LC-MS

Gary A. Valaskovic¹, Mike S. Lee^2
1New Objective Inc, Woburn, MA, ²Milestone Development Services, Newtown, PA

Method to Optimize Sensitivity and Throughput on a Hybrid Quadrupole-TOF Instrument for LC/MS-Based Peptide and Proteomic Analysis

Joseph Bonapace¹, Jennifer Rutherford¹, James P. Murphy III², Gary A. Valaskovic^2
1Charles River Proteomic Services, Worcester, MA ²New Objective Inc, Woburn, MA

Automated Orthogonal Control System for Electrospray Ionization Mass Spectrometry

Gary A. Valaskovic¹, James P. Murphy III¹, Mike S. Lee^2
1New Objective Inc, Woburn, MA, ²Milestone Development Services, Newtown, PA

Minimizing Post-Column Band Broadening in Nanobore LC-MS/MS for Bottom-up Proteomic Applications

Andreas Huhmer¹, James P. Murphy III², Gary A. Valaskovic^2
1Thermo Electron Corp., San Jose, CA, ²New Objective, Inc., Woburn, MA

Fundamental Studies on the Application of Nanobore LC-MS for the Analysis of Small Drug Molecules

Mike S. Lee¹, James P. Murphy III², Gary A. Valaskovic^2
1Milestone Development Services, Newtown, PA, ²New Objective, Inc., Woburn, MA

Automated ESI Control on Variable-Flow Gradient Nanobore LC-MS

James P. Murphy III¹, Gary A. Valaskovic¹, Karen M. Hahnenberger², David W. Neyer^2
1New Objective Inc, Woburn, MA, ²Eksigent Technologies, Livermore, CA

An Automated Control System for Nano-Electrospray

James P. Murphy III, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Fabrication and Use of 20 µm ID Nanobore Columns

Gary A. Valaskovic and James P. Murphy III
New Objective, Inc., Woburn, MA
Not available for download

Trap/Column Combination to Optimize Sensitivity and Sequence Coverage

James P. Murphy III and Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Variable Flow Gradient LC-MS: Spray Mode Signal Affects and Control

Mike S. Lee, Gary A. Valaskovic, and James P. Murphy III
New Objective, Inc., Woburn, MA

A Comprehensive Study on Nanobore Injection Methods for Proteomics

James P. Murphy III, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Practical High Sensitivity LC-MS: Fundamentals, Challenges, and Prospects

Gary A. Valaskovic
New Objective, Inc, Woburn, MA

Improved Nanospray Emitter Coatings for Nanospray LC-MS (Abstract only)

James P. Murphy III and Gary A. Valaskovic
New Objective, Inc, Woburn, MA

Taylor Cone Stability and ESI Performance for LC-MS at Low Flow Rates (Abstract only)

¹Mike S. Lee, ²James P. Murphy III, ²Gary A. Valaskovic
¹Milestone Development Services, Newtown, PA ²New Objective Inc, Woburn, MA

Orthogonal Control Systems for ESI-MS and Nanobore LC-MS (Abstract only)

¹Gary A. Valaskovic, ¹James P. Murphy III, ²Mike S. Lee
¹New Objective Inc, Woburn, MA ²Milestone Development Services, Newtown, PA

Comparison of Nanobore LC/MS Injection Methods for Proteomics

James P. Murphy III and Gary A. Valaskovic
New Objective, Inc., Woburn