Posters Presented at ASMS 2009 - Philadelphia, PA

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Towards Robust Quantitative MRM Plasma Analysis using Nanobore Liquid Chromatogrpahy Through Improves Nanoelectrospray Performance
Susan e. Abbatiello¹, Amanda Berg², Gary Valaskovic², Steven A. Carr ^1
1The Broad Institute, Cambridge, MA; ¹New Objective, Woburn, MA
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Minimization of Atmospheric Background Contaminants in Nanoelectrospray: Identification and Optimization
Benjamin Ngo, Gary A. Valaskovic
New Objective Inc, Woburn, MA
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Fundamental Studies on the Electrophoretic Enrichment of Peptides in Clinical Samples for Quantification using nanoLC-MS/MS
Jeremy L. Norris¹, Gary Valaskovic², Mike S. Lee³, Chuck Witkowski^1
1Protein Discovery, Inc., Knoxville, TN; ²New Objective Inc, Woburn, MA; ³Milestone Development Services, Newtown, PA
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Top Down Proteomics Using Online Polymer Reversed Phase (PLRP) Nanocapillary-LC-Coupled Fourier Transform Mass Spectrometry
Adaikkalam Vellaichamy¹, John Kellie¹, John C. Tran¹, Ji Eun Lee¹ Jaeyun Sung¹,
Nathan D. Price¹, Gary Valaskovic², Neil L. Kelleher^1
1University of Illinois, Urbana, IL; ²New Objective Inc, Woburn, MA
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Comparison of Chromatographic Media for Nano ESI LC-MS/MS: Applications to Biomarker Discovery
Michael Gardner¹, Megan Rowland², Jonathan L. Bundy¹, James Stephenson¹, Gary Valaskovic³, Mike S. Lee^4
1Research Triangle Institute, RTP, NC; ²RTI International, Cary, NC; ³New Objective Inc, Woburn, MA; ⁴Milestone Development Services, Newtown, PA
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Ruggedness of Nanobore LCMS for Qualitative and Quantitative Biomarker Analysis using an Automated Emitter Positioning and Rinsing System
Amanda Berg, Carla Marshall-Waggett, Gary Valaskovic
New Objective Inc, Woburn, MA
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Improved Throughput for Clinical Proteomics Using a Dual Channel NanoLC-Nanospray Source
Cheryl F. Lichti¹, Benjamin Ngo², Marjorie N. Case¹, R.Reid Townsend¹, Gary A. Valaskovic^2
1Washington University School of Medicine, St. Louis, MO; ²New Objective Inc, Woburn, MA
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Comparison of Conventional, Sub-2-µm, and Superficially Porous (Fused-Core) Particles for Long and Short Gradient Nanobore LC/MS
Robert Moody¹, Gary A. Valaskovic², Mike S. Lee^3
1MAC-MOD Analytical, Chadds Ford, PA; ²New Objective Inc, Woburn, MA; ³Milestone Development Services, Newtown, PA
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Temperature Control Effects in Packed Emitter Nanobore LC-MS/MS for Protein Digest Analysis
Mike S. Lee¹, Lee Swadey², Amanda Berg², Gary A. Valaskovic^2
1Milestone Development Services, Newtown, PA; ²New Objective Inc, Woburn, MA
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Spatial Mapping of Analyte Ion Intensity of Nanoelectrospray in the Plume-Inlet Region
Gary A. Valaskovic¹, Mike S. Lee^2
1New Objective Inc, Woburn, MA; ²Milestone Development Services, Newtown, PA
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High Sensitivity Nanoscale Multidimensional LC/MS Analysis of Phosphopeptides
Scott Ficarro¹, Yi Zhang², Feng Zhou¹, Bob Cardoza¹, Guillame Adelmont¹, Manor Askenazi², Amanda Berg³, Gary A. Valaskovic³, Jared Marto^1
1Dana-Farber Cancer Institute, Boston, MA; ²Dana-Farber Cancer Institute and Hebrew University, Boston, MA; ³New Objective Inc, Woburn, MA
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Posters Presented at ABRF 2009 - Memphis, Tennessee

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Imaging of Nanoelectrospray Spray Current with an Automated Digital Control Positioning System
Gary A. Valaskovic¹, Mike S. Lee^2
1New Objective, Woburn, MA; ²Milestone Development Services, Newtown, PA
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Nanoelectrospray Ruggedness for Qualitative and Quantitative Biomarker Analysis Using an Automated Emitter Positioning and Rinsing System
Amanda Berg, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA
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Minimization of Atmospheric Background Contaminants in Off- and Online Nanoelectrospray: Identification and Optimization
Benjamin Ngo, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA
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Posters Presented at ASMS 2008 - Denver, Colorado

Optimization of Nanoelectrospray with Plasma Derived Samples for Qualitative and Quantitative Biomarker Analysis
Jeffrey Wynn, Amanda Berg, Gary A. Valaskovic
New Objective Inc, Woburn, MA
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Performance Comparison of Standard Electrospray vs. Nanospray for the Quantification of Nucleopeptides and their Phosphorolated Metabolites
John D. Lennon¹, Ken Lewis¹, Gary A Valaskovic^2
1OpAns, LLC, Durham, NC; ²New Objective Inc, Woburn, MA
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Optimization of Novel Fused-Core Silica Particle Nanobore Columns Using a Pump-Switching Motif for Mass Spectrometry
Robert Moody¹; Gary A. Valaskovic²; Mike S. Lee^3
1MAC-MOD Analytical, Chadds Ford, Pennsylvania; ²New Objective, Inc., Woburn, MA; ³Milestone Development Services, Newtown, PA
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High-Sensitivity Nanocapillary Chromatography Combined With Active Background Ion Reduction Increases Signal-to-Noise Ratios, Sensitivity, and Overall System Performance
John M. Neveu¹, Bogdan A. Budnik¹, Gary A. Valaskovic², William S. Lane^1
1Harvard University, Cambridge, MA; ²New Objective, Inc., Woburn, MA
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Maximizing Performance of sub-2 µm Packed nanobore LC Columns: Minizing Extra-Column Variance
Carla J . Marshall-Waggett, Amanda L. Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA
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Investigation of Elastomer Core Compression Effect on Minimizing Trapping Column Band Broadening
Amanda L. Berg, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA
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Measurement of Testosterone in Human Urine by Direct Injection Using nano-Pump Switching with Nanospray Tandem Mass Spectrometry
Daniel Magiera¹, Mike S. Lee², Gary A. Valaskovic^3
1Molecular MS Diagnostics, LLC, Cranston, RI; ²Milestone Development Services, Newtown, PA; ³New Objective, Inc., Woburn, MA
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Imaging of Nanoelectrospray Spray Current with an Automated Digital Control Positioning System
Gary A. Valaskovic¹, Mike S. Lee^2
1New Objective, Woburn, MA; ²Milestone Development Services, Newtown, PA
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Improving the Sensitivity of LC/MS Assays for Low-Abundance Protein Biomarkers: Bridging the Gap with ELISA
Lee W. Ott¹, Michael J. Berna¹, Gary A. Valaskovic², Bradley L. Ackermann^1
1Eli Lilly & Co., Greenfield, IN; ²New Objective, Inc., Woburn, MA
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Posters Presented at ASMS 2007 - Indianapolis, Indiana

Correlating Extended Dynamic Range with Nanospray Flow Rate Measurement for Optimal ESI-MS Performance
Carla J. Marshall-Waggett, Christopher J. Toher, Gary A. Valaskovic
New Objective, Woburn, MA
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Inline Electrospray Current Conductivity Detection for Characterization of Mobile Phase Composition and Gradient Delay
Adam W. Perala, Gary A. Valaskovic
New Objective Inc, Woburn, MA
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Efficient Column-to-Column Coupling with Multiple Columns for Enhanced nLC-ESI-MS Separation
Christopher Toher, Adam W. Perala, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA
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A Novel Fused-core Silica Particle Based Nanobore Column for Ultra-high Performance Nanoelectrospray Mass Spectrometry
Robert Moody¹; Gary A. Valaskovic²; Mike S. Lee^3
1MAC-MOD Analytical, Chadds Ford, Pennsylvania, ²New Objective, Inc., Woburn, MA, ³Milestone Development Services, Newtown, PA
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Ultra-Performance Peptide Analysis via Nanobore LC-ESI-MS with Sub-2µm Particles
John M. Neveu¹, William S. Lane¹, Gary A. Valaskovic^{2 1}Harvard University, Cambridge, MA;
²New Objective, Inc., Woburn, MA
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Digitized Nanobore LC-MS: An Automated Control System with Emitter Divert for Column-Switching
Mike S. Leesup>1, Gary A. Valaskovic^{2 1}Milestone Development Services, Newtown, PA, ²New Objective, Inc., Woburn, MA
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Evaluation of Fluoropolymer Frit Performance in High-pH Nano-ESI Applications
Jeffrey Wynn, Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective Inc, Woburn, MA
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On-line 1D and 2D nanoLC-ESI-MS using 10-µm-i.d. Porous Layer Open Tubular Polystyrene-divinylbenzene Columns for Ultrasensitive Proteomic Analysis
Quanzhou Luo¹, Guihua Yue¹, Gary A. Valaskovic², Ye Gu¹, Dongdong Wang¹, Shiaw-Lin Wu¹, Barry L. Karger^1
1The Barnett Institute, Northeastern University, Boston, MA, ²New Objective, Inc., Woburn, MA
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Posters Presented at ABRF 2007 - Tampa, Florida

Inline Electrospray Current Conductivity Detection for Characterizing Mobile Phase Composition and Gradient Delay
Gary A. Valaskovic, Adam W. Perala, Christopher J. Toher
New Objective Inc, Woburn, MA
Size: 684 K | Download Poster

Long- & Mixed-Column Nanobore Chromatography for Complex Proteomic Analysis
Christopher J. Toher, Adam W. Perala, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA
Size: 936 K | Download Poster

Examination of Nanobore Capillary Trapping Column Geometries for Analysis of Peptide Mixtures
John M. Neveu¹, Christopher J. Toher², William S. Lane¹, Gary A. Valaskovic^2
1Harvard University, Cambridge, MA   ²New Objective Inc, Woburn, MA
Size: 968 K | Download Poster

Rapid Nanobore LC-ESI-MS Peptide Analysis Using Sub-3 µm-Diameter Resins
Adam W. Perala, Christopher J. Toher, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA
Size: 516 K | Download Poster

Posters Presented at ASMS 2006 - Seattle, Washington

Optimization of Nanobore Capillary Trapping Column Geometries for Analysis of Peptide Mixtures
John M. Neveu¹, Christopher J. Toher², William S. Lane¹, Gary A. Valaskovic^2
1Harvard University, Cambridge, MA   ²New Objective Inc, Woburn, MA
Size: 1.2 MB | Download Poster

High-Pressure, Transparent Microfluidic Coupling Method for Nanobore LC-MS
Jeffrey J. Wynn, Christopher J. Toher, Adam W. Perala, Gary A. Valaskovic
New Objective Inc, Woburn, MA
Size: 536 K | Download Poster

Digitized Nanobore LC-MS Control System with Integrated Emitter Divert & Rinse for Improved Nanospray Performance
James L. Stephenson¹, Jonathan L. Bundy¹, Gary A. Valaskovic², Mike S. Lee^3
1RTI International, Research Triangle Park, NC   ²New Objective Inc, Woburn, MA   ³Milestone Development Service, Inc., Newtown, PA
Size: 552 K | Download Poster

Offline Nano-ESI Phosphopeptide Analysis with TiO₂, and ZrO₂ Wall-Coated Trap'nTips™
Christopher J. Toher¹, Adam W. Perala¹, Ashok K. Shukla², Gary A. Valaskovic^1
1New Objective Inc, Woburn, MA   ²Glygen, Inc., Columbia, MD
Size: 332 K | Download Poster

Posters Presented at ABRF 2006 - Long Beach, California

Offline Nano-ESI Phosphopeptide Analysis with Carbon, TiO₂, and ZrO₂
Wall-Coated Trap'nTips™
Christopher J. Toher¹, Adam W. Perala¹, Ashok K. Shukla², Gary A. Valaskovic^1
1New Objective Inc, Woburn, MA  ²Glygen, Inc., Columbia, MD
Size: 272 K | Download Poster

PicoClear™ Union Technology Applications for Nano ESI-MS Molecules
Carla J. Marshall-Waggett, Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective Inc, Woburn, MA
Size: 460 K | Download Poster

Evaluation of Fluoropolymer Frit Performance in High-pH Nano-ESI Applications
Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective Inc, Woburn, MA
Size: 480 K | Download Poster

Posters Presented at ASMS 2005 - San Antonio, Texas

Automated Orthogonal ESI Control System with Optimized Plume-Inlet Positioning
Mike S. Lee ¹, Gary A. Valaskovic^2
1Milestone Development Services, Inc.  ²New Objective Inc, Woburn, MA
Size: 432 K | Download Poster

Sample Purification for Static Nanospray MS Using Wall-Coated Pipette Trap'nTips™
Christopher J. Toher ¹, Adam W. Perala ¹, Ashok K. Shukla ², Gary A. Valaskovic^1
1New Objective Inc, Woburn, MA  ²Glygen, Inc., Columbia, MD
Size: 636 K | Download Poster

Enhanced Nanobore LC-MS Using Methanol Gradient Elution with Peptide Mixtures
Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective, Inc., Woburn, MA
Size: 756 K | Download Poster

TIC-Mediated Flow Rate Variation for Flow-Injection Nanospray Analysis
Carla J. Waggett¹, David Neyer², Adam W. Perala¹, Christopher J. Toher¹, Gary A. Valaskovic^1
2New Objective, Inc., Woburn, MA  ²Eksigent Technologies, Livermore, CA
Size: 328 K | Download Poster

Charged Droplet Solvent Extraction from Surfaces: Desoprtion Electrospray Ionization of Small Drug Molecules (Abstract only)
Gary A. Valaskovic¹, Mike S. Lee^2
1New Objective Inc, Woburn, MA  ²Milestone Development Services, Inc.
Size: 150 K | Download Abstract

Posters Presented at ASMS 2004 - Nashville, TN

Method to Optimize Sensitivity and Throughput on a Hybrid Quadrupole-TOF Instrument for LC/MS-Based Peptide and Proteomic Analysis
Joseph Bonapace¹, Jennifer Rutherford¹, James P. Murphy III², Gary A. Valaskovic^2
1Charles River Proteomic Services, Worcester, MA ²New Objective Inc, Woburn, MA
Size: 500 K | Download Poster

Automated Orthogonal Control System for Electrospray Ionization Mass Spectrometry
Gary A. Valaskovic¹, James P. Murphy III¹, Mike S. Lee^2
1New Objective Inc, Woburn, MA ²Milestone Development Services, Newtown, PA
Size: 664 K | Download Poster

Minimizing Post-Column Band Broadening in Nanobore LC-MS/MS for Bottom-up Proteomic Applications
Andreas Huhmer¹, James P. Murphy III², Gary A. Valaskovic^2
1Thermo Electron Corp., San Jose, CA ²New Objective, Inc., Woburn, MA
Size: 744 K | Download Poster

Fundamental Studies on the Application of Nanobore LC-MS for the Analysis of Small Drug Molecules
Mike S. Lee¹, James P. Murphy III², Gary A. Valaskovic^2
1Milestone Development Services, Newtown, PA ²New Objective, Inc., Woburn, MA
Size: 492 K | Download Poster

Automated ESI Control on Variable-Flow Gradient Nanobore LC-MS
James P. Murphy III¹, Gary A. Valaskovic¹, Karen M. Hahnenberger², David W. Neyer^2
1New Objective Inc, Woburn, MA ²Eksigent Technologies, Livermore, CA
Size: 700 K | Download Poster

Posters Presented at ASMS 2003 - Montreal, Quebec, Canada

Fabrication and Use of 20 µm ID Nanobore Columns
Gary A. Valaskovic and James P. Murphy III
New Objective, Inc., Woburn, MA
Abstract only available
Size: 144 K| Download Abstract

Trap/Column Combination to Optimize Sensitivity and Sequence Coverage
James P. Murphy III and Gary A. Valaskovic
New Objective, Inc., Woburn, MA
Size: 764 K| Download Poster

Variable Flow Gradient LC-MS: Spray Mode Signal Affects and Control
Mike S. Lee, Gary A. Valaskovic, and James P. Murphy III
New Objective, Inc., Woburn, MA
Size: 1.4 MB| Download Poster

Poster Presented at ABRF 2003

A Comprehensive Study on Nanobore Injection Methods for Proteomics
James P. Murphy III and Gary A. Valaskovic
New Objective, Inc., Woburn, MA
Size: 2 MB | Download Poster

Industrial Seminar Presented at HPCE 2003

Practical High Sensitivity LC-MS: Fundamentals, Challenges, and Prospects
Gary A. Valaskovic
New Objective, Inc, Woburn, MA
Size: 5.8 MB | Download PPT Presentation

Technical Abstracts from ASMS 2002

Improved Nanospray Emitter Coatings for Nanospray LC-MS
James P. Murphy III and Gary A. Valaskovic
New Objective, Inc, Woburn, MA
Size: 76 K | Download Abstract

Taylor Cone Stability and ESI Performance for LC-MS at Low Flow Rates
¹Mike S. Lee, ²James P. Murphy III, ²Gary A. Valaskovic
¹Milestone Development Services, Newtown, PA ²New Objective Inc, Woburn, MA
Size: 1.2 MB | Download Abstract

Orthogonal Control Systems for ESI-MS and Nanobore LC-MS
¹Gary A. Valaskovic, ¹James P. Murphy III, ²Mike S. Lee
¹New Objective Inc, Woburn, MA ²Milestone Development Services, Newtown, PA
Size: 188 K | Download Abstract