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Chromatography Chemistries

ABOVE: HALO's fused-core particles are manufactured by
"fusing" a porous silica layer onto a solid silica particle. The
shorter diffusion path of HALO particles reduces axial dispersion
and minimizes peak broadening. This is particularly advantageous
for chromatographic separations of molecules larger than ~400 DA and for
high speed separations using higher mobile phase flow velocities.
BELOW: Chromatograms for HALO packed trap/
analytical column combination for 30 fmol and 300
fmol injections of BSA digest to show injection
repeatability. A 25 min. linear gradient from 2%B to
50% B was used. Three blank injections were performed between the
30 and 300 fmol runs.

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- Provides hyper-fast separations with modest backpressure
- Reliable, rugged performance
- Fast separations without ultra-high pressure
- Base-deactivated for excellent peak shape
- Available in all PicoFrit and IntegraFrit Columns
- HALO C8, C18
- HALO RP
- HALO HILIC
HALO® columns are manufactured using Fused-Core® particle technology
that was specially developed to deliver hyper-fast chromatographic
separations while avoiding the reliability issues so often associated
with fast HPLC.
HALO's ability to provide hyper-fast separations
comes not only from the small 2.7 µm particle size but
also from the unique Fused-Core particle technology that creates
a 0.5 µm porous shell fused to a solid core particle enabling
HALO columns to deliver over twice the separating power of a
column packed with 5 µm
particles. HALO
columns maintain their resolving power at high flow rates. This
means that shorter columns and higher flow rates can be used
to achieve remarkably fast high resolution separations.
Stationary Phase Support
- Ultra-pure, “Type B” silica
- 1.7 µm solid core particle with a 0.5 µm porous silica layer fused
to the surface
- 150 m2/gram surface area
- 90 Å pore size
Bonded Phase
- Monomeric bonding chemistry
- Densely bonded phase
- Maximized endcapping
- C18: Octadecyldimethylsilane, 3.5 µmoles/m2
- C8: Octyldimethylsilane, 3.7 µmoles/m2
- pH Range: 2 to 9
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HALO and Fused-Core are registered trademarks of Advanced Materials Technology
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PROTEOPEP™ II
ProteoPep™ II produces sharper peaks with less noise than ProteoPep™.
- C18, 300 Å pore material excellent for both hydrophobic and hydrophilic peptides
- Exceptional peak shape for peptide analysis with trace (0.005%) or no TFA
- High peak counts high resolution
- Peptide mapping and purification
- Synthetic peptide purification
- 5 µm particle size
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More Chemistries
ProteoPep™ III
From New Objective, Inc.
• C18, 80 Å
• pH range: 2-9
• Carbon load: 10%
• Double-endcapped; USP L1
• Excellent choice for low-pH applications
• Low back pressures
• 1.8 particle size
Inertsil® ODS-3
From GL Sciences, Inc.
• C18, 100 Å
• pH range: 2-7.5
• Carbon load: 15%
• Endcapped
• Predictable selectivity with very low operating pressure
• 3 µm, 5 µm particle size
Inertsil® ODS-4
From GL Sciences, Inc.
• C18, 100 Å
• Carbon load: 11%
• Endcapped; USP L1
• Ideal for 100% aqueous conditions
• 2 µm, 3 µm, 5 µm particle sizes
Titansphere®
From GL Sciences, Inc.
• TiO2, 100 Å
• Excellent for phophoylated peptide extraction
• Ideal for both basic and acidic conditions
• 5 µm particle size
Monitor™
From Orochem Technologies, Inc.
• C18, 250 Å
• pH range: 2-8
• Base deactivated
• Low back pressure
• Improved peak shape for basic and acidic compounds
• 3 µm, 5 µm particle sizes
Gazelle™
From Orochem Technologies, Inc.
• C18, 100 Å
• pH range: 2-8
• Base deactivated
• Low back pressure
• Ideal for fast gradient analysis
• 1.7 µm particle size
BioBasic®
From Thermo Fisher Scientific
• C18, 300 Å, Carbon load: 9%
• C8, 300 Å, Carbon load 5%
• C4, 300 Å, Carbon load 4%
• Endcapped
• Highly deactivated
• Ideal for proteins and larger peptides
• High peak capacity
• 5 µm particle size
AQUASIL
From Thermo Fisher Scientific
• C18, 100 Å
• Immune to retention loss by pure water
• Deactivated acids or bases
• Good performance with low TFA
• 5 µm particle size
Standard Chemistries
ProteoPep™
From New Objective, Inc.
• C18, 300 Å
• Exceptional peak shape for peptide analysis with trace (0.005%) or no TFA
• Recommended for intact proteins
• 5 µm particle size
Strong Cation Exchange
From New Objective, Inc.
• SCX, 85Å
• 5 µm particle size
Biphasic PicoFrit® Columns
- Analyzes hundreds of proteins in a single run
- Combines high-quality cation exchange material with ProteoPep™ II C18 or any of our other premier chemistries
Biphasic PicoFrit® Column
(Washburn, M.P., D. Wolters, J.R. yates. Nature Biotechnology 19 (2001): 242-247.)
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