Chromatography Chemistries

ABOVE: HALO's fused-core particles are manufactured by "fusing" a porous silica layer onto a solid silica particle. The shorter diffusion path of HALO particles reduces axial dispersion and minimizes peak broadening. This is particularly advantageous for chromatographic separations of molecules larger than ~400 DA and for high speed separations using higher mobile phase flow velocities. BELOW: Chromatograms for HALO packed trap/ analytical column combination for 30 fmol and 300 fmol injections of BSA digest to show injection repeatability. A 25 min. linear gradient from 2%B to 50% B was used. Three blank injections were performed between the 30 and 300 fmol runs.

Provides hyper-fast separations with modest backpressure Reliable, rugged performance Fast separations without ultra-high pressure Base-deactivated for excellent peak shape Available in all PicoFrit and IntegraFrit Columns HALO C8, C18 HALO RP HALO HILIC HALO® columns are manufactured using Fused-Core® particle technology that was specially developed to deliver hyper-fast chromatographic separations while avoiding the reliability issues so often associated with fast HPLC. HALO's ability to provide hyper-fast separations comes not only from the small 2.7 µm particle size but also from the unique Fused-Core particle technology that creates a 0.5 µm porous shell fused to a solid core particle enabling HALO columns to deliver over twice the separating power of a column packed with 5 µm particles. HALO columns maintain their resolving power at high flow rates. This means that shorter columns and higher flow rates can be used to achieve remarkably fast high resolution separations. Stationary Phase Support Ultra-pure, “Type B” silica 1.7 µm solid core particle with a 0.5 µm porous silica layer fused to the surface 150 m2/gram surface area 90 Å pore size Bonded Phase Monomeric bonding chemistry Densely bonded phase Maximized endcapping C18: Octadecyldimethylsilane, 3.5 µmoles/m2 C8: Octyldimethylsilane, 3.7 µmoles/m2 pH Range: 2 to 9

PROTEOPEP™ II ProteoPep™ II produces sharper peaks with less noise than ProteoPep™. C18, 300 Å pore material excellent for both hydrophobic and hydrophilic peptides Exceptional peak shape for peptide analysis with trace (0.005%) or no TFA High peak counts — high resolution Peptide mapping and purification Synthetic peptide purification 5 µm particle size

More Chemistries

ProteoPep™ III
From New Objective, Inc.
• C18, 80 Å
• pH range: 2-9
• Carbon load: 10%
• Double-endcapped; USP L1
• Excellent choice for low-pH applications
• Low back pressures
• 1.8 particle size

Inertsil® ODS-3
From GL Sciences, Inc.
• C18, 100 Å
• pH range: 2-7.5
• Carbon load: 15%
• Endcapped
• Predictable selectivity with very low operating pressure
• 3 µm, 5 µm particle size

Inertsil® ODS-4
From GL Sciences, Inc.
• C18, 100 Å
• Carbon load: 11%
• Endcapped; USP L1
• Ideal for 100% aqueous conditions
• 2 µm, 3 µm, 5 µm particle sizes

Titansphere®
From GL Sciences, Inc.
• TiO2, 100 Å
• Excellent for phophoylated peptide extraction
• Ideal for both basic and acidic conditions
• 5 µm particle size

Monitor™
From Orochem Technologies, Inc.
• C18, 250 Å
• pH range: 2-8
• Base deactivated
• Low back pressure
• Improved peak shape for basic and acidic compounds
• 3 µm, 5 µm particle sizes

Gazelle™
From Orochem Technologies, Inc.
• C18, 100 Å
• pH range: 2-8
• Base deactivated
• Low back pressure
• Ideal for fast gradient analysis
• 1.7 µm particle size

BioBasic®
From Thermo Fisher Scientific
• C18, 300 Å, Carbon load: 9%
• C8, 300 Å, Carbon load 5%
• C4, 300 Å, Carbon load 4%
• Endcapped
• Highly deactivated
• Ideal for proteins and larger peptides
• High peak capacity
• 5 µm particle size

AQUASIL
From Thermo Fisher Scientific
• C18, 100 Å
• Immune to retention loss by pure water
• Deactivated acids or bases
• Good performance with low TFA
• 5 µm particle size

Standard Chemistries

ProteoPep™
From New Objective, Inc.
• C18, 300 Å
• Exceptional peak shape for peptide analysis with trace (0.005%) or no TFA
• Recommended for intact proteins
• 5 µm particle size

Strong Cation Exchange
From New Objective, Inc.
• SCX, 85Å
• 5 µm particle size

Biphasic PicoFrit^® Columns

• Analyzes hundreds of proteins in a single run
• Combines high-quality cation exchange material with ProteoPep™ II C18 or any of our other premier chemistries

Biphasic PicoFrit^® Column

(Washburn, M.P., D. Wolters, J.R. yates. Nature Biotechnology 19 (2001): 242-247.)