PicoFrit^® Robustness

The study of global proteomics requires robust performance on a 24/7 basis. With the right sample preparation, PicoFrit^® columns provide high predictability without compromising sensitivity.

Injection #31 - DAY TWO Plasma membrane challenged with insulin In gel digestion of slice no. 30 of 64 75 µm 4x10 cm C18 PicoFrit® column with 300 µm, 1 mm C18 Trap Cartridge on Micromass® Q-ToF™		Injection #881 - ONE MONTH LATER Smooth endoplasmic reticulum, aqueous phase In gel digestion of slice no. 45 of 92 Over 30 days of continuous, 24/7 operation (Data courtesy Daniel Boismenu, McGill University)

PicoFrit^® Specifications

• Column IDs 75 µm and 50 µm, tip IDs of 15 µm and 10 µm
• Packed with a variety of high-performance, reverse-phase, HPLC packings
• Short columns (1 cm) for sample concentration/desalting procedures
• Medium columns (5 cm) for rapid-gradient LC-MS
• Long columns (10 cm) for gradient LC-MS
• Biphasic columns for shotgun digests