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Select the Right Tip for Your Mass SpectrometerFor optimal results and without the need of additional equipment some mass spectrometers require a specific size emitter. The most common systems and their respective emitter sizes are:
GlassTips™
EconoTips™
QuartzTips™
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INSIDER TIPHOW TO FILL
GLASS TIPS The optimal taper shape of GlassTips™ makes sample filling a snap, featuring a "self-filling" design. Normally troublesome air bubbles (which can cause "vapor lock" in other tips) have no negative effect on performance.
Load your sample into a suitable pipette tip. Shown here is a 2 µL sample in a recommended GELoader™ pipette tip, using a 0.1 to 10 µL pipettor.
Insert the GELoader™ tip into the distal end of the GlassTip™. Press slowly on the plunger to inject your sample. This is important with viscous peptide and protein samples.
As seen in the photo, the sample has been loaded into the far (distal) end of the tip. Capillary action will guide your sample into the tapered region of the emitter. There is no need to centrifuge your sample down into the nanospray tip.
Within a minute or two, capillary action has "pumped" your sample into the tip region. The GlassTip™ is now ready to use for mounting on your nanospray source. |
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