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INSIDER TIPS

HOW TO FILL GLASS PICOTIPS™

The optimal taper shape of GlassTips™ makes sample filling a snap, featuring a "self-filling" design. Normally troublesome air bubbles (which can cause "vapor lock" in other tips) have no negative effect on performance.

Microscopic photo of pipette with droplet being inserted into back of GlassTip

Sample loaded in pipette tip

Load your sample into a suitable pipette tip. Shown here is a 2 µL sample in a recommended GELoader™ pipette tip, using a 0.1 to 10 µL pipettor.

Microscopic photo of sample being loaded from pipette into GlassTip

Loading sample into GlassTip™

Insert the GELoader™ tip into the distal end of the GlassTip™. Press slowly on the plunger to inject your sample. This is important with viscous peptide and protein samples.

Microscopic photo of the sample being drawn from the back of the GlassTip into the tip via capillary action

GlassTip™ and sample

As seen in the photo, the sample has been loaded into the far (distal) end of the tip. Capillary action will guide your sample into the tapered region of the emitter. There is no need to centrifuge your sample down into the nanospray tip.

High-magnification view of filled tip

Within a minute or two, capillary action has "pumped" your sample into the tip region. The GlassTip™ is now ready to use for mounting on your nanospray source.

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