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Choosing the Right Tip
STEP 2: I am looking
for PicoTips® for offline, static nanospray
Offline PicoTip® Emitters
Borosilicate glass and quartz PicoTips® are an improved version
of the traditional nanospray ionization emitters. Their special taper makes them
virtually self-filling and their specified tip opening eliminates breaking open
the tips prior to use.
The proper OD for the emitter is determined by the manufacturer of your nanospray
source. The Micromass (Waters) ZSpray™ NanoFlow™ uses PicoTips with
a 1.0 mm OD while the ABI Mariner™ NanoLINK™, Bruker NanoSpray™,
MSD Proteomics/Protana NanoES, and Thermo Finnigan Nanospray Ion sources take
PicoTips with a 1.2 mm OD.
Determine your target flow rate then locate the corresponding tip size on the
table below. EconoTips™ have
a 1 µm ID tip and are perfect for routine sampling with a standard single-layer
conductive coating. GlassTips™ are
available in 2 and 4 µm tip IDs, with or without a durable, inert, multilayer
conductive coating (U.S. Patent 5,788,166) and undergo a rigorous inspection
by video microscopy. Select QuartzTips™ for
samples that are sodium sensitive or need an emitter with better resistance to
high pH solvents.
| Tip
Size vs. Flow Rate |
| Tip Size,
ID (µm) |
Flow Rate*
(nL/min) |
| 1 |
20-80 |
| 2 |
20-80 |
| 4 |
40-100 |
Approximate range of flow rates over which the tips will generate a stable electrospray
plume without sheath gas assistance. Actual performance may differ considerably
caused by variations in experimental parameters (mobile phase composition, applied
voltage, source design etc.)
Next:
Choose a coating option
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